Effect of progesterone on intracellular Ca2+ homeostasis in human myometrial smooth muscle cells.
نویسندگان
چکیده
Although it is well known that progesterone alters uterine contractility and plays an important role in maintenance of pregnancy, the biochemical mechanisms by which progesterone alters uterine contractility in human gestation are less clear. In this investigation we sought to identify progesterone-induced adaptations in human myometrial smooth muscle cells that may alter Ca2+signaling in response to contractile agents. Cells were treated with vehicle or the progesterone analog medroxyprogesterone acetate (MPA) for 5 days, and intracellular free Ca2+ concentration ([Ca2+]i) was quantified after treatment with oxytocin (OX) or endothelin (ET)-1. OX- and ET-1-induced increases in [Ca2+]iwere significantly attenuated in cells pretreated with MPA in a dose-dependent manner. Progesterone receptor antagonists prevented the attenuated Ca2+ transients induced by MPA. ETA and ETB receptor subtypes were expressed in myometrial cells, and treatment with MPA resulted in significant downregulation of ETAand ETB receptor binding. MPA did not alter ionomycin-stimulated increases in [Ca2+]iand had no effect on inositol trisphosphate-dependent or -independent release of Ca2+ from internal Ca2+ stores. We conclude that adaptations of Ca2+ homeostasis in myometrial cells during pregnancy may include progesterone-induced modification of receptor-mediated increases in [Ca2+]i.
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Effect of progesterone on intracellular Ca21 homeostasis in human myometrial smooth muscle cells
Fomin, Victor P., Blair E. Cox, and R. Ann Word. Effect of progesterone on intracellular Ca21 homeostasis in human myometrial smooth muscle cells. Am. J. Physiol. 276 (Cell Physiol. 45): C379–C385, 1999.—Although it is well known that progesterone alters uterine contractility and plays an important role in maintenance of pregnancy, the biochemical mechanisms by which progesterone alters uterine...
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ورودعنوان ژورنال:
- The American journal of physiology
دوره 276 2 Pt 1 شماره
صفحات -
تاریخ انتشار 1999